Composite

Part:BBa_K4497033:Design

Designed by: Till Gundlach   Group: iGEM22_Munich   (2022-10-09)


tTA Induceable 2xmCherry T2A EYFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 465
    Illegal EcoRI site found at 2020
    Illegal XbaI site found at 484
    Illegal SpeI site found at 490
    Illegal SpeI site found at 2089
    Illegal PstI site found at 471
    Illegal PstI site found at 2299
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 465
    Illegal EcoRI site found at 2020
    Illegal SpeI site found at 490
    Illegal SpeI site found at 2089
    Illegal PstI site found at 471
    Illegal PstI site found at 2299
    Illegal NotI site found at 476
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 465
    Illegal EcoRI site found at 2020
    Illegal BamHI site found at 589
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 465
    Illegal EcoRI site found at 2020
    Illegal XbaI site found at 484
    Illegal SpeI site found at 490
    Illegal SpeI site found at 2089
    Illegal PstI site found at 471
    Illegal PstI site found at 2299
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 465
    Illegal EcoRI site found at 2020
    Illegal XbaI site found at 484
    Illegal SpeI site found at 490
    Illegal SpeI site found at 2089
    Illegal PstI site found at 471
    Illegal PstI site found at 2299
    Illegal AgeI site found at 597
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

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References